Flowchart of Gram stain morphology that can be used to identify aerobic Gram-positive cocci.

Aerobic Gram-Positive Rods Flowchart.

Flowchart (with images) distinguishing alpha and gamma-hemolytic streptococci on blood agar plates.

Summary of characteristics (with images) that can be used to distinguish alpha streptococci from streptococcus pneumoniae on blood agar plates.

Summary of characteristics (with images) that can be used to identify Bacillus spp.

Flowchart that can be used to identify bacteria by using Gram-positive stain.

As part of the Microbiology Curriculum, this course is designed to familiarize you with basic culture media and the techniques used for microorganism identification in the microbiology laboratory. You will explore various culture media, learn to inoculate media through the use of a four quadrant streaking procedure, and recognize common problems when performing microbiological culture techniques.

As part of the Microbiology Curriculum, this course is designed to familiarize you with basic culture media and the techniques used for microorganism identification in the microbiology laboratory. You will explore various culture media, learn to inoculate media through the use of a four quadrant streaking procedure, and recognize common problems when performing microbiological culture techniques.

As part of the Microbiology Curriculum, this course is designed to familiarize you with basic culture media and the techniques used for microorganism identification in the microbiology laboratory. You will explore various culture media, learn to inoculate media through the use of a four quadrant streaking procedure, and recognize common problems when performing microbiological culture techniques.

As part of the Microbiology Curriculum, this course is designed to familiarize you with basic culture media and the techniques used for microorganism identification in the microbiology laboratory. You will explore various culture media, learn to inoculate media through the use of a four quadrant streaking procedure, and recognize common problems when performing microbiological culture techniques.

As part of the Microbiology Curriculum, this course is designed to familiarize you with basic culture media and the techniques used for microorganism identification in the microbiology laboratory. You will explore various culture media, learn to inoculate media through the use of a four quadrant streaking procedure, and recognize common problems when performing microbiological culture techniques.

As part of the Microbiology Curriculum, this course is designed to familiarize you with basic culture media and the techniques used for microorganism identification in the microbiology laboratory. You will explore various culture media, learn to inoculate media through the use of a four quadrant streaking procedure, and recognize common problems when performing microbiological culture techniques.

Before a cell divides and DNA is passed from one cell to another, a complex process occurs. The DNA strands unwind and separate. Each strand makes a complementary strand by adding the appropriate nucleotides. As a result, there are now two double-stranded DNA molecules in the nucleus that contain the same information. This process is known as replication. This animation will describe the complex process in detail.

Deoxyribonucleic acid (DNA) is a complex molecule of many components. These components can be divided into four main groups: basic elements, nucleosides, nucleotides, and nucleic acids. This video will describe how these four groups build upon each other to create the DNA molecule.

RNA is similar in structure to DNA but is involved in different cellular functions. RNA contains the same basic elements of DNA but with three major differences in its structure. This video will describe these three differences. The video will also touch on the different types of RNA found in a cell and their functions.

In performing molecular procedures in the laboratory, it is essential that you keep contamination down to a minimum. Contamination can affect the results of your test. This video will discuss the necessary steps that should be used in reducing contamination.

Column-based extraction is a method that employs selective binding of nucleic acid to a solid matrix, such as silica that is packed in a column. This video will show this procedure to remove nucleic acid from a specimen.

Gel electrophoresis is a method used for separation of nucleic acid using a porous gel matrix depending on the size of the nucleic acid. This video will show how this procedure is performed.

Organic extraction is a method used to separate nucleic acid and other cellular components based on their differential solubility. This method uses a mixture of organic solvents, phenol and chloroform, to extract unwanted cellular components from nucleic acid. This video will show how the procedure is performed.

Polymerase chain reaction or PCR is a technique for amplifying specific DNA fragments from a DNA template. PCR happens in three basic steps: denaturation, annealing, and extension. This video will show the steps in amplifying specific DNA fragments for the PCR technique.

Detection of PCR products in real time can be accomplished by using fluorescent dyes or probes. Fluorescently labeled probes detect the amount of specific double-stranded DNA sequences. While fluorescent dyes detect only the amount of double-stranded DNA, in this video we are going to look at the principles behind fluorescent dye-based detection method.

Flowchart (with images) that can be used to identify beta-hemolytic streptococci on blood agar plates.

Images of positive and negative biochemical tests for Gram negative organisms.

Images showing biochemical tests for identifying Gram- positive organisms.

Guide that facilitators/mentors can use to oversee the completion of Gram-negative laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.

Hands-on Gram-negative exercises that learners can use along with equipment in their laboratory to reinforce concepts from associated eLearning course.

Guide that facilitators/mentors can use to oversee the completion of Gram-positive laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.

Instructions (with images) for care and maintenance of a microscope.

List of questions to consider while following a specimen from collection through microbiological testing.

Descriptions (with images) showing colony morphology characteristics.

This template is intended to help supervisors or training evaluators assess PPE competency in clinical and public health laboratory settings. The goal is to determine whether employees can appropriately select, don, doff, and dispose of PPE according to site—and activity-specific risk assessments.

Table that describes various general media used in a public health or clinical microbiology laboratory.

This job aid reviews basic information about diagnostic sensitivity and specificity for clinical laboratory testing.

Flowchart for identifying E. coli (non-stool).

A microscope is a very important instrument in the laboratory. It is necessary to focus your microscope when viewing specimens to perform a proper analysis and give an accurate diagnosis. This job aid gives a brief overview of how to focus on a specimen using a compound microscope. It demonstrates the steps in focusing a compound light microscope from 10X to 100X. The use of immersion oil for the 100X lens is also specifically shown.

Glossary of terms and references for the Gram Negative Organism ID course.

List of supplies and reagents needed to conduct a Gram stain and instructions on how to conduct a Gram staining procedure.

Images depicting Gram stain results and cell shapes and arrangements.

Flowchart for identifying lactose negative Gram-negative rods.

Flowchart for identifying lactose positive Gram-positive rods.

Gram-Negative Rods Non-Stool Pathogens Flowchart.

Flowchart for identifying Gram-negative rods stool pathogens.

This microlearning focuses on the laboratory technique known as the Gram stain, which is commonly used to differentiate between bacterial species that are Gram-positive and Gram-negative.

This microlearning focuses on the laboratory technique known as the catalase test, which is commonly used to differentiate between bacterial species you will learn the proper techniques with the slide method and the tube method.

This microlearning focuses on the laboratory technique known as the Motility Test, which is commonly used to differentiate between bacterial species by detecting if a bacterium is motile due to the presence of flagella.

This microlearning teaches the proper techniques to be used while performing the Oxidase Test, which is one of several tests that is used to identify Gram-negative bacteria.

Summary of characteristics (with images) that can be used to identify listeria monocytogenes.

This template is intended to help supervisors document the PPE training of employees in clinical and public health laboratory settings. Employees must be trained on PPE usage before they are allowed to perform work in areas requiring PPE. The types of PPE and their processes (e.g., donning and doffing) will be determined by the laboratory’s site-or procedure-specific risk assessment.

This template is intended to help supervisors or evaluators of PPE programs in clinical and public health laboratory settings. The goal is to determine whether appropriate policies and procedures are in place to enable employees to safely and effectively use PPE. This template should be customized to address site-specific needs.

Instructions and list of supplies (with images) for preparing a wet mount.

Demonstration of the proper technique for performing an India ink stain.

Demonstration of how to perform a potassium hydroxide or, KOH preparation, for detecting the presence of yeast in a specimen under a microscope.

Demonstration of how to perform a wet mount using a bright-field microscope and the proper equipment.

Guide that facilitators/mentors can use to oversee the completion of routine microscopy procedures laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.

This job aid contains hands-on routine microscopy exercises that learners can use along with equipment in their laboratory to reinforce concepts from the associated eLearning course.

This job aid will familiarize learners with the major sections of the microscope.

Flowchart (with images) that can be used to identify staphylococci.

Chart and images that can be used to interpret TSI reactions.

Yersinia pestis Characteristics Chart.

Yersinia pestis Differentiation Chart.

Flowchart for identifying Y. pestis.