Laboratory Fundamentals

Courses

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Public Health Laboratories (PHL) 101

Public health laboratories focus on diseases and the health status of population groups. They perform limited diagnostic testing, reference testing, and disease surveillance.

These materials provide an overview of public health laboratory core functions and services.

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Routine Microscopy Procedures

This course is designed to explore the processes, procedures, and techniques necessary for completing routine microscopic examinations of laboratory specimens. 

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OneLab VR: Packing and Shipping Dangerous Goods

The goal for this scenario is for the user to properly triple package the sample and safely transport it to the Specimen Receiving area for air shipping, then apply the proper marks and labels in the proper locations to the package and seal it.

OneLab VR Tutorial

OneLab VR: Tutorial Scenario

OneLab VR encompasses 12 different laboratories in a 50,000-square-foot virtual facility created for clinical and public health laboratory training. It also includes over 100 (and counting) laboratory-specific pieces of hardware and equipment.

Events

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ISO 35001:2019 Biorisk Management for Laboratories

With the complexities of laboratory environments combined with emerging biological agents, it is

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How to Plan for B. pseudomallei Exposure Cases - A Laboratory Perspective

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CLIA Proficiency Testing (PT) Final Rule, CMS-3355-F

This presentation will provide information on the CLIA Proficiency Testing (PT) Final Rule which ...

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Life of a Test Method—Validation, Verification, and Managing Quality Part 1

This session will support learners in better understanding the Food and Drug Administration and C...

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Public Health Laboratories (PHL) 101

Public health problems are diverse and can include infectious diseases, chronic...

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When Receiving Samples from Patients Suspected to be Infected with Ebola: What’s the Plan?

Learn how clinical and public health laboratories collaborate with healthcare teams to provide th...

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I’m a Frontline Facility – How Can I Safely Test Clinical Specimens From a Suspected Ebola Patient

Join us for our next OneLab Network Event in which will give an overview of the current Sudan Ebo...

Job Aids

Routine Microscopy Procedures Student Laboratory Exercises Job Aid

   

CLIA Proficiency Testing (PT) Final Rule, CMS-3355-F Frequently Asked Questions

 

Fundamentals of Laboratory Safety - Laboratory Hazards Scenario

 

Fundamentals of Laboratory Safety - Introduction to Laboratory Safety

 

Fundamentals of Laboratory Safety - Emergency Procedures and Reporting Scenario

 

Fundamentals of Laboratory Safety - Incident or Near Miss Scenario

 

Fundamentals of Laboratory Safety - Laboratory Work Practices Scenario

 

Fundamentals of Laboratory Safety - Near Miss Scenario

 

Fundamentals of Laboratory Safety - Risk Management Scenario

 

Fundamentals of Handling Compressed Gas Cylinders – Setting Regulator Pressure

 

Routine Microscopy – Direct Smear

   

Routine Microscopy – Gram Stain Procedure

 

Routine Microscopy – India Ink

 

Routine Microscopy – KOH Preparation

 

Routine Microscopy – Wet Mount

 

Basic Microscopy – Ocular Micrometer Calibration

 

Basic Microscopy – Microscope Components

 

Basic Microscopy – Köhler Illumination

 

Basic Microscopy – Focusing the Microscope

 

Basic Microscopy – Cleaning the Microscope

 

Basic Microscopy – Setting Up the Microscope

 

Laboratory Supply Chain Frequently Asked Questions

 

Bacillus anthracis (Anthrax) Characteristics Chart

   

Common Safety Work Practices in the Laboratory

   

Laboratory Onboarding Template (LOT)

   

Fundamentals of Working Safely in a Biological Safety Cabinet (BSC): Safe Use of a BSC

 

Fundamentals of Working Safely in a Biological Safety Cabinet (BSC): Cleaning Up a Spill in a BSC

 

Yersinia pestis Differentiation Chart

   

Presumptive Identification Chart for Francisella tularemia

   

Francisella tularensis Identification Flowchart

   

Francisella tularensis Characteristics Chart

   

B. pseudomallei Identification Flowchart

   

B. pseudomallei Characteristics Chart

   

B. mallei Identification Flowchart

   

B. mallei Characteristics Chart

   

Table 2: Summary of Recommended Biosafety Levels for Infectious Agents

   

Brucella spp. Characteristics Chart

   

Brucella spp. Identification Flowchart

   

Bacillus anthracis Identification Flowchart

   

Routine Microscopy Procedures Laboratory Exercises Facilitator Guide

   

Preparing a Wet Mount

   

Smear Preparation

   

Staphylococci Identification Chart

   

Listeria Monocytogenes Identification Chart

   

Biochemical Tests for Gram- Positive Organism Identification

   

Beta-Hemolytic Streptococci on Blood Agar Plates Identification Chart

   

Basic Bacterial Identification from Gram-Positive Stain

   

Bacillus spp. Identification Chart

   

Alpha Streptococci vs Streptococcus pneumoniae Identification Chart

   

Alpha or Gamma-Hemolytic Streptococci Blood Agar Plates Flowchart

   

Aerobic Gram-Positive Rods Flowchart

   

Gram-Negative Rods Stool Pathogens Flowchart

   

Gram-Negative Rods Non-Stool Pathogens Flowchart

   

Gram-Negative Rod (GNR) Stool Pathogens Lactose Positive Flowchart

   

Gram-Negative Rod (GNR) Stool Pathogens Lactose Negative Flowchart

   

Gram Stain Cell Morphology

   

Gram Stain

   

Escherichia coli (Non-Stool) Identification Chart

   

Colonial Characteristics

   

Biochemical Tests for Gram Negative Organism Identification

   

Aerobic Gram-Negative Rods Flowchart

   

Sections of the Microscope

   

Care and Maintenance of the Microscope

   

Laboratory Communications Toolkit: Quick Reference Resource for Creating Meaningful Messages

   

Basic Molecular Biology: Nucleic Acid Extraction – Organic Extraction

 

Basic Molecular Biology: Nucleic Acid Extraction – Magnetic Bead-Based Extraction

 

Basic Molecular Biology: Nucleic Acid Extraction – Liquid Phase Extraction

 

Basic Molecular Biology: Nucleic Acid Extraction – Gel Electrophoresis

 

Basic Molecular Biology: Nucleic Acid Extraction – Column-Based Extraction

 

Basic Molecular Biology: Laboratory Practice – The Laboratory Working Areas

 

Fundamentals of Working Safely in a Biological Safety Cabinet (BSC): Completing Work in a BSC

 

Fundamentals of Working Safely in a Biological Safety Cabinet (BSC): Preparing for Work in a BSC

 

Fundamentals of Working Safely in a Biological Safety Cabinet (BSC): Factors Affecting BSC Airflow

 

Provider Performed Microscopy Procedures

 

Vital Records Matrix

   

Procedural Steps for Identifying and Prioritizing Essential Functions

   

Identification of Essential and Nonessential Laboratory Functions

   

Examples of Purpose

   

Alternate Site Familiarization Program

   

Checklist for Safe Use of Biological Safety Cabinets

   

Laboratory Medicine Best Practices (LMBP) Data Abstraction Tool: LMBP Data Abstraction Coding Manual

   

Study Quality Appraisal Checklist

   

Results Log – Quantitative Test

   

Results Log – Qualitative Test

   

Results Log with QC – Quantitative Test

   

Results Log with QC – Qualitative Test

   

Results Log for Multiple Test

   

Pretesting Task Checklist

   

Instructions for Performing External Control

   

Instructions for Logging or Recording Results

   

Hand Hygiene Job Aid

   

Glove Removal Job Aid

   

Blood and Body Fluid Exposure

   

Nasopharyngeal (NP) Specimen Collection

 

Nasal Mid-Turbinate Specimen Collection

 

COVID-19 Point-of-Care Batch Testing Tips

 

Step 4: Labeling, Marking, and Documenting Requirements Job Aid