Basic Microscopy – Köhler Illumination

To make an accurate diagnosis, it is necessary to visualize samples as clearly as possible. Köhler illumination is a light microscopy where the user aligns the illuminating optics to produce a uniform background, maximize contrast, minimize artifacts, and reduce stray light. This video is a brief tutorial on how to set Köhler illumination on a compound microscope to get a brilliant image. Please note that the ability to adjust the condenser aperture or field aperture (diaphragm) may vary by manufacturer. Consult your manual for additional instructions.

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Training Laboratory microscopy microscope compound microscope light microscope Köhler Illumination Köhler setup Köhler alignment light source knob field diaphragm stage controls oculars bright field biomedical science laboratory science histological techniques histology condenser lens objective lens uneven illumination inhomogeneous illumination transmitted light microscopy phase microscopy DIC microscopy aperture brightness illumination focus stage slide biology microbiology cell biology molecular biology cell and molecular biology