Basic Molecular Biology: PCR and Real-Time PCR – RT-PCR Fluorescent Probe-Based Detection

Detection of PCR products in real time can be accomplished by using fluorescent dyes or probes. Fluorescently labeled probes detect the amount of specific double-stranded DNA sequences. While fluorescent dyes detect only the amount of double-stranded DNA, in this video we are going to look at the principles behind fluorescent dye-based detection method.