Artwork for Basic Molecular Biology Module 4: PCR and Real-Time PCR
1.0 Contact Hour
1.0 Hour
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Basic Molecular Biology Module 4: Polymerase Chain Reaction (PCR) and Real-Time PCR


Molecular techniques have been widely used in clinical diagnosis, e.g., diagnosing disease, predicting disease course, and identifying infectious agents. This basic molecular biology course series will introduce the scientific background for molecular diagnosis, the principles of molecular biology laboratory practice, and common methods.

This basic-level eLearning course provides information on the principle of PCR and real-time PCR. Topics covered include PCR steps, PCR product analysis, and real-time PCR characteristics.


This online course is designed for public health and clinical laboratory staff, and persons interested in PCR and real-time PCR techniques.


Course content is closed captioned, where applicable, and optimized for a screen reader.


At the end of this course, learners should be able to:

  • Explain the basic steps involved in PCR
  • Identify the components of PCR, reverse transcription PCR, and PCR product analysis
  • Recognize the characteristics of real-time PCR
  • Identify the techniques used to detect products in real-time PCR
  • Differentiate the nucleic acid quantification processes used in real-time PCR
  • Explain the roles of PCR controls  
Continuing Education (CE)

The Centers for Disease Control and Prevention, Division of Laboratory Systems is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program. This course is approved for 1.0 contact hours.

P.A.C.E.® course number: 288-011-23.