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1.0 Contact Hour(s)
1.0 Hour
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Fundamentals of Handling Compressed Gas Cylinders Safely


The safe handling of compressed gas cylinders is critical for laboratory staff. Proper handling of compressed gases and gas cylinders creates a safe laboratory.

This course introduces clinical and public health laboratory professionals to the physical and chemical hazards associated with compressed gas and compressed gas cylinders, control measures to mitigate risks, and how to work safely with compressed gases and gas cylinders in the laboratory.


This basic-level course is designed for clinical and public health laboratory staff, safety professionals, and others interested in the safe use of compressed gases and compressed gas cylinders.


Course content is closed captioned, where applicable, and optimized for a screen reader.


At the end of this course, learners should be able to:

  • Recognize the physical and chemical hazards of compressed gas cylinders and the associated risks when working with them.
  • Identify work practices and control measures to safely store, transport and handle compressed gas cylinders.
  • Describe methods for controlling the risks based on the specific type of gas.
  • Describe how to safely select and attach the correct gas regulator.
  • List the steps to follow when removing the gas regulator.
  • Identify how to test for a leak in the system when attaching the regulator to the cylinder.
Continuing Education (CE)

The Centers for Disease Control and Prevention, Division of Laboratory Systems is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program. This course is approved for 1.0 contact hours. P.A.C.E.® course number: 288-014-23