Chart listing characteristics of Bacillus Anthracis (Anthrax).

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Flowchart for identifying aerobic Gram-negative rods or Gram-negative coccobacilli.

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Aerobic Gram-Positive Rods Flowchart.

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This job aid aims to provide lab audiences with general knowledge and tools to conduct a Hotwash and After-Action Report after an incident, near miss, training exercise, or emergency response event.

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Flowchart (with images) distinguishing alpha and gamma-hemolytic streptococci on blood agar plates.

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Summary of characteristics (with images) that can be used to distinguish alpha streptococci from streptococcus pneumoniae on blood agar plates.

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Form used to provide an overview of important points to consider when conducting business from an Emergency Relocation Site.

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This job aid accompanies the Assessing Laboratory Training Needs course. It provides a summary of how to apply CDC Quality Training Standard #1, along with exercises to perform a training needs assessment for your laboratory training.

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Job aid for the specific steps needed to safely attach the regulator to a compressed gas cylinder.

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Chart listing characteristics of B. mallei.

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Flowchart used to identify or rule out B. mallei.

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Chart listing characteristics of B. pseudomallei.

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Flowchart used to rule out or refer B. pseudomallei.

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Flowchart used to rule out or refer Bacillus anthracis.

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Summary of characteristics (with images) that can be used to identify Bacillus spp.

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Flowchart that can be used to identify bacteria by using Gram-positive stain.

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Guide that facilitators/mentors can use to oversee the completion of basic culture media exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.

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This job aid contains a list of terms commonly associated with basic culture media and isolation techniques and a brief description of each term.

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This job aid contains hands-on basic culture media and isolation techniques exercises that learners can use along with equipment in their laboratory to reinforce concepts from the associated eLearning course.

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This job aid contains a list of supplies needed and instructions for performing a four-quadrant streak on an Agar plate to obtain isolated colonies from a specimen.

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This job aid includes examples of recommended media that can be plated to recover microorganisms from a particular body site. The selection of types and forms can vary depending on the laboratory protocol and availability.

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Hands-on microscopy laboratory exercises that learners can use along with equipment in their laboratory to reinforce concepts from associated eLearning course.

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Flowchart (with images) that can be used to identify beta-hemolytic streptococci on blood agar plates.

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Images of positive and negative biochemical tests for Gram negative organisms.

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Images showing biochemical tests for identifying Gram- positive organisms.

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Guide that facilitators/mentors can use to oversee the completion of Gram-negative laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.

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Hands-on Gram-negative exercises that learners can use along with equipment in their laboratory to reinforce concepts from associated eLearning course.

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Guide that facilitators/mentors can use to oversee the completion of Gram-positive laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.

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This resource familiarizes laboratory professionals with how to read a Gram stain, colonial characteristics, biochemical tests used to identify Gram-positive microorganisms, and commonly used testing algorithms.

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This job aid details the use of standard precautions when cleaning up blood or other body fluids, including handwashing, rinsing of mucous membrane or eyes after exposure, treating skin punctures, and reporting exposure, as well as glove safety.

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This job aid can help guide employee conversations with their supervisor for specific information or guidance concerning training, administrative controls, and incident reporting.

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This job aid is a reference document for the required steps to take following a blood or OPIM exposure incident.

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This job aid is a reference document for items that should be included in a biological spill kit and the appropriate steps for cleaning up a blood or OPIM spill.

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Chart listing characteristics of Brucella spp.

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Flowchart used to rule out or refer Brucella spp.

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Size is one of the most important physical features when identifying and characterizing an organism in the lab. The exact size of an organism can be determined using a calibrated ocular micrometer.

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Instructions (with images) for care and maintenance of a microscope.

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Compressed Gas Association Valve Outlet Listing

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Job aid for the steps needed to safely check the compressed gas cylinder for leaks.

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Checklist of activities/tasks needed to safely use biological safety cabinets.

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Featuring the top questions asked during OneLab’s popular 2023 webinar, with answers from Penny Keller, BS MB(ASCP), Centers for Medicare and Medicaid Services.

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List of questions to consider while following a specimen from collection through microbiological testing.

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Descriptions (with images) showing colony morphology characteristics.

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This job aid details the use of standard disinfectants and antiseptics in the laboratory, including instructions for the preparation of a 10% bleach solution.

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This job aid contains personal safety work and general safety work practices for working in the Laboratory.

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This template is intended to help supervisors or training evaluators assess PPE competency in clinical and public health laboratory settings.

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This job aid accompanies the Ensuring Accuracy and Relevancy of Laboratory Training course. It is a practical tool designed to help laboratory trainers align their training content with their learning objectives.

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Worksheet to list the various communications systems a laboratory uses, the current providers, restoration contacts, and backup modes of communication.

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A notification list used to determine who has the authority to activate the continuity of operations plan based on predetermined triggers and how others in the organization will be notified of the decision to activate.

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Worksheet to list all facilities in which the organization operates, and designations for which facilities will be needed in an emergency or disaster situation.

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Worksheet to list essential functions, personnel needed, and other important information needed to maintain the continuity of the essential function when moving to an alternate worksite.

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Flowchart to assist in determining whether to activate a continuity of operations plan.

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Worksheet to list an organization’s critical functions, the primary authority (e.g., decision-making, procurement), and the order of succession in case the primary authority is unavailable.

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Guide for recording priorities and essential functions for laboratory continuity of operations.

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Worksheet to determine overall staffing needs for essential laboratory functions.

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Instructions, guidance, and sample text for the development of continuity plans and programs.

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This infographic includes general information to improve COVID-19 specimen handling and processing for more accurate results.

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This product includes crisis response information and resources for laboratories to use in the event of various crisis events.

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Table used to determine whether to activate a continuity of operations plan based on the impact various emergencies may have on the laboratory.

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This resource discusses what virtual training is and explores the features of popular online learning platforms.

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This job aid accompanies the Designing for Usability and Accessibility of Laboratory Training course. It provides a summary of how to apply CDC’s Quality Training Standard #5, along with exercises to incorporate usability and accessibility practices into your laboratory training.

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This job aid accompanies the Developing S.M.A.R.T. Laboratory Training Learning Objectives course. It provides a summary of how to apply CDC Quality Training Standard #2, along with exercises to develop S.M.A.R.T. learning objectives for your laboratory training.

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Template that planners can use to detail how essential laboratory operations will continue in the event the primary operating facility is incapacitated and personnel are unavailable or incapable of deploying to the alternate facility.

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This job aid reviews basic information about diagnostic sensitivity and specificity for clinical laboratory testing.

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The Diagnostic Stewardship toolkit was created to help organizations form diagnostic stewardship teams and apply guiding principles. Diagnostic Stewardship supports ordering the right test for the right patient at the right time to improve patient outcomes.   

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Chart listing characteristics of biothreat agents and similar organisms.

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Table listing characteristics of biothreat agents and similar organisms.

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Chart differentiating Brucella spp. from other urea-positive, oxidase-positive gram-negative coccobacilli.

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Donning and doffing Personal Protective Equipment is essential to keeping laboratorians and the public safe from possible contamination. This video focuses on donning and doffing a laboratory coat, gloves, and safety glasses with wrap around sides.

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This video demonstrates the donning and doffing of the following PPE: Gown, Gloves, Disposable Face Mask, and Face Shield. The doffing portion of this video demonstrates removing the gown and gloves.

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This video demonstrates the donning and doffing of the following PPE: Gown, Gloves, Disposable Face Mask, and Face Shield. The doffing portion of this video demonstrates removing the gown and gloves.

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This job aid is based on the fictitious EdU scenario in the Laboratory Risk Management course and is intended only for the purpose of this risk management training. Please reference your laboratory's policies and procedures for site-specific risks and considerations.

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Table to list contact information of emergency personnel and other important contacts.

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This resource guide includes available biological, chemical, and radiological resources for laboratories to reference during an emergency.

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This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, and the proper response to incidents.

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This job aid accompanies the Ensuring Accuracy and Relevancy of Laboratory Training course. It provides a summary of how to apply CDC Quality Training Standard #3, along with exercises to ensure the accuracy and relevancy of your laboratory training.

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Flowchart for identifying E. coli (non-stool).

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Examples of statements of applicability and scope for continuity of operations plans.

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Examples of executive summaries for continuity of operations plans.

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Examples of purpose statements for laboratory continuity of operations plans.

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Guide that facilitators/mentors can use to oversee the completion of basic microscopy laboratory exercises. The guide contains objectives, laboratory setup, a supply list, and laboratory exercises, instructions, and an answer key as well as job aids.

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A microscope is a very important instrument in the laboratory. It is necessary to focus your microscope when viewing specimens to perform a proper analysis and give an accurate diagnosis. This job aid gives a brief overview of how to focus on a specimen using a compound microscope.

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This is a sample formaldehyde job hazard analysis that can be used to document the chemical’s hazards and controls by job step. It also contains information about emergency procedures, training, and review and monitoring.

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Descriptions and images depicting characteristics of Francisella tularensis.

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Flowchart describing major characteristics in identifying Francisella tularensis.

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Reference Document for the 12 Quality System Essentials (QSE), that describe the foundational elements of laboratory Quality Management Systems (QMS). 

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Fact sheet listing common questions and answers about genetic testing and results.

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This job aid details glove safety in the laboratory, including how to choose disposable gloves, precautions while wearing protective gloves, and the safe removal and disposal of gloves.

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This is a sample glutaraldehyde job hazard analysis that can be used to document the chemical’s hazards and controls by job step. It also contains information about emergency procedures, training, and review and monitoring.

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Glossary of terms and references for the Gram Negative Organism ID course.

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List of supplies and reagents needed to conduct a Gram stain and instructions on how to conduct a Gram staining procedure.

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Images depicting Gram stain results and cell shapes and arrangements.

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Flowchart for identifying lactose negative Gram-negative rods.

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Flowchart for identifying lactose positive Gram-positive rods.

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Gram-Negative Rods Non-Stool Pathogens Flowchart.

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Flowchart for identifying Gram-negative rods stool pathogens.

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This job aid details the steps for proper hand hygiene in the laboratory, including instructions for cleaning the hands with water or with an alcohol-based sanitizer.

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Table listing examples of potential laboratory threats (e.g., earthquake or fire), mitigation measures, and risks.

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Fact sheet containing basic information about molecular genetic testing and results.

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This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, and the proper response to incidents.

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This document contains the qualifications required for a clinical consultant in a laboratory performing high complexity testing.

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This document contains the qualifications required for a cytology general supervisor in a laboratory performing high complexity testing.

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This document contains the qualifications required for a cytotechnologist in a laboratory performing high complexity testing.

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This document contains the qualifications required for a general supervisor in a laboratory performing high complexity testing.

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This document contains the qualifications required for a laboratory director in a laboratory performing high complexity testing.

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This document contains the qualifications required for a technical supervisor in a laboratory performing high complexity testing. Cytology technical supervisor qualifications are also included in the cytology section.

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This document contains the qualifications required for testing personnel in a laboratory performing high complexity testing.

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Worksheet to help identify essential and non-essential laboratory functions.

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This job aid accompanies the Improving Laboratory Training Through Evaluation course. It provides a summary of how to apply CDC’s Quality Training Standard #6, along with exercises to develop an evaluation plan for your laboratory training.

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This resource discusses what learning engagement is, the benefits that come from it, and the practices that you can take to increase learner engagement.

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Instructions for, and examples of, logging or recording results for qualitative tests, quantitative tests, quality control – qualitative tests, quality control – quantitative tests, and multiple tests.

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Instructions and list of supplies (with images) for examining specimens for yeast.

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To make an accurate diagnosis, it is necessary to visualize samples as clearly as possible. Köhler illumination is a light microscopy where the user aligns the illuminating optics to produce a uniform background, maximize contrast, minimize artifacts, and reduce stray light.

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Clinical and public health laboratories can use this toolkit to translate complex information into user-friendly messages for various audiences.

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This job aid covers essential topics for correctly delegating responsibilities to CLIA laboratory personnel and for identifying responsibilities a laboratory director must retain.

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An infographic and summary that depicts the Laboratory Information Management System (LIMS) as a “system of systems”.

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Manual summarizing rules and guidelines for using a standardized abstraction form to record evidence for systematic reviews for best practices in laboratory medicine.

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The goal of this product is to provide laboratory supervisors and staff with an interactive workbook to share safety protocols and common work practices in the laboratory.

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The goal of this product is to provide laboratory supervisors and staff with an interactive workbook to share safety protocols and common work practices in the laboratory.

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This Laboratory Onboarding Template (LOT) is a central location for laboratory-specific information and foundational training resources to rapidly onboard new hires or surge support staff from multidisciplinary backgrounds.

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The Laboratory Personal Protective Equipment (PPE) Toolkit is a guide to resources on using PPE in clinical and public health laboratories.

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This quick guide contains links to the resources included in the Laboratory Personal Protective Equipment (PPE) Toolkit.

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The Supply Chain Frequently Asked Questions Job Aid is a follow-up resource from the “Supply Chain Lessons Learned and Q&A” OneLab network event held on October 28, 2021. 

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This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, and the proper response to incidents.

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An example of a laboratory test request form.

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Summary of characteristics (with images) that can be used to identify listeria monocytogenes.

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This flowchart outlines the qualifications necessary for a clinical consultant to comply with CLIA regulations related to moderate complexity testing as specified in 42 CFR 493.1417.

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This flowchart outlines the qualifications necessary for a laboratory director to manage and direct laboratory personnel and the performance of moderate complexity tests as specified by CLIA regulations in 42 CFR 493.1405.

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This flowchart details the qualifications required for a technical consultant to ensure compliance with CLIA regulations for moderate complexity testing as specified in 42 CFR 493.1411.

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This flowchart describes the qualifications required for testing personnel to perform moderate complexity testing as specified by CLIA regulations in 42 CFR 493.1423.

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This infographic is a resource/tool to improve nasal mid-turbinate swab specimen collection for SARS-CoV-2 testing.

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This infographic is a resource/tool to improve nasopharyngeal swab specimen collection for SARS-CoV-2 testing.

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Worksheet to list individuals and organizations to notify in the event of an emergency and the person responsible for notifying them.

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Worksheet to notify staff and others when the laboratory’s continuity of operations plan is implemented.

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In this scenario, the user will demonstrate the proper steps to safely load, operate, and unload an autoclave. The user is tasked with decontaminating biohazardous waste. The autoclave scenario is performed in the Autoclave room.

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In this scenario, the user is tasked with centrifuging specimens with a swinging bucket centrifuge. The user must select the correct tubes, properly balance, and load the swinging bucket centrifuge.

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In this scenario, the user is tasked with packing and next-day air shipping a Category B specimen of Salmonella during a nationwide outbreak and sending it to another laboratory for further evaluation.

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This job aid accompanies the Laboratory Opportunities for Learner Engagement in Laboratory Training course.

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Worksheet to list essential functions, key personnel, and their successors.

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This job aid details the steps to follow when performing external control testing using known positive or negative clinical samples. It includes steps to follow.

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This template is intended to help supervisors document the PPE training of employees in clinical and public health laboratory settings. Employees must be trained on PPE usage before they are allowed to perform work in areas requiring PPE.

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This template is intended to help supervisors or evaluators of PPE programs in clinical and public health laboratory settings. The goal is to determine whether appropriate policies and procedures are in place to enable employees to safely and effectively use PPE.

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Table to list names and contact information for each employee.

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The Point of Care Testing (POCT) Personal Protective Equipment (PPE) Toolkit is a guide to resources on using PPE in POCT settings.

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Instructions and list of supplies (with images) for preparing a wet mount.

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Flowchart used to rule out or refer Francisella tularensis.

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Tool to help identify and prioritize essential laboratory functions.

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Regulatory requirements, resources including forms and examples, and images of common microscopic findings for the nine specific microscopic examinations that may be performed under a Certificate of PPM Procedures.

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This job aid details the steps to follow when performing quality control testing to ensure tests perform as expected and alert the user when problems occur.

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Worksheet to record changes to a continuity of operations plan outside the official plan review cycle.

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Job aid for the specific steps needed to safely remove the regulator from a compressed gas cylinder.

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This job aid is a downloadable and printable results log for reporting qualitative testing.

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This job aid is a downloadable and printable results log for reporting quantitative testing with quality control.

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This job aid is a downloadable and printable results log for reporting multiple types of testing.

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This job aid is a downloadable and printable results log for reporting qualitative testing with quality control.

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This job aid is a downloadable and printable results log for reporting quantitative testing with quality control.

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This risk example resource is based on a fictitious scenario and is intended for use alongside the risk management training. 

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Guide that facilitators/mentors can use to oversee the completion of routine microscopy procedures laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.

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This job aid contains hands-on routine microscopy exercises that learners can use along with equipment in their laboratory to reinforce concepts from the associated eLearning course.

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This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, and the proper response to incidents.

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This job aid serves as a quick reference guide for personnel trained to pack and ship suspected or confirmed SARS-CoV-2 specimens.

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This job aid will familiarize learners with the major sections of the microscope.

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This job aid is a downloadable and printable example of a checklist that details four main areas of focus when completing pretesting tasks: 1. preparation of the work area; 2. checking and recording temperatures; 3. maintaining equipment; and 4. preparing materials for testing.

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Job aid for the specific steps needed to safely set the regulator pressure for a compressed gas cylinder.

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Example of typical shipping papers used for ground transport of Category A substances.

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Job aid for the specific steps needed to safely shut down and remove the regulator on a compressed gas cylinder.

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Instructions and list of supplies (with images) describing the process of making a smear preparation.

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This job aid defines the criteria to properly ship biological specimens to the Centers for Disease Control and Prevention (CDC).

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Flowchart (with images) that can be used to identify staphylococci.

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Decision tree to help classify substance if shipping by motor vehicle courier/ground in accordance with Hazardous Materials Regulations (HMR).

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Decision tree to help determine the classification for substance if shipping by air.

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Reference document for packing Category A, Category B, and Exempt Human and Exempt Animal Specimens.

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Reference table for labeling, marking, and documenting requirements for the various classifications and modes of transport.

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Checklist containing study quality components.

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Table summarizing recommended biosafety levels for infectious agents.

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This job aid details the steps to follow when performing and logging temperature control checks in the laboratory. It includes instructions for recording temperatures and an example of a completed monthly refrigerator/freezer temperature log.

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Worksheet that planners can use to list testing, training, and exercise requirements and the frequency that each should occur.

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This job aid provides tips for Selecting and Using Personal Protective Equipment (PPE).

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Worksheet for recording details about essential files, records, and databases that need to be accessed after the laboratory’s continuity of operations plan has been implemented.

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This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, waste disposal best practices, and the proper response to incidents.

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Yersinia pestis Differentiation Chart.

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