Hands-on microscopy laboratory exercises that learners can use along with equipment in their laboratory to reinforce concepts from associated eLearning course.
Compound microscopes require regular cleaning to help ensure accurate diagnoses and prevent damage to the microscope. However, microscopes are delicate instruments that must be handled with care.
A microscope is a very important instrument in the laboratory. It is necessary to focus your microscope when viewing specimens to perform a proper analysis and give an accurate diagnosis. This video gives a brief overview of how to focus on a specimen using a compound microscope.
To make an accurate diagnosis, it is necessary to visualize samples as clearly as possible. Köhler illumination is a light microscopy where the user aligns the illuminating optics to produce a uniform background, maximize contrast, minimize artifacts, and reduce stray light.
The most common type of microscope is a compound light microscope. It contains two or more lenses and uses visible light to produce a two-dimensional image of an object viewed through the oculars.
Size is one of the most important physical features when identifying and characterizing an organism in the lab. The exact size of an organism can be determined using a calibrated ocular micrometer.
Microscopes are important pieces of equipment used in the management of patient care. Compound microscopes have mechanical and optical components essential to their function and use. It is important to set up microscopes for optimal viewing every time it is used.
The creation of RNA is made possible by a process called bacterial transcription. Through transcription the information contained in a section of DNA is replicated to form a new piece of messenger RNA (mRNA). This video will cover the three steps to create mRNA.
In performing molecular procedures in the laboratory, it is essential that you keep contamination down to a minimum. Contamination can affect the results of your test. This video will discuss the necessary steps that should be used in reducing contamination.
Column-based extraction is a method that employs selective binding of nucleic acid to a solid matrix, such as silica that is packed in a column. This video will show this procedure to remove nucleic acid from a specimen.
Gel electrophoresis is a method used for separation of nucleic acid using a porous gel matrix depending on the size of the nucleic acid. This video will show how this procedure is performed.
When performing liquid phase nucleic acid extraction, a method called alcoholic precipitation is used where contaminants such as organic solvents salts and proteins are removed from nucleic acid in a solution. This video will show how the procedure is performed.
Magnetic bead-based extraction is a method that utilizes small particles with a paramagnetic core that binds to nucleic acid. This video will show how the procedure is performed.
Organic extraction is a method used to separate nucleic acid and other cellular components based on their differential solubility. This method uses a mixture of organic solvents, phenol and chloroform, to extract unwanted cellular components from nucleic acid.
Size is one of the most important physical features when identifying and characterizing an organism in the lab. The exact size of an organism can be determined using a calibrated ocular micrometer.
This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, and the proper response to incidents.
Guide that facilitators/mentors can use to oversee the completion of basic microscopy laboratory exercises. The guide contains objectives, laboratory setup, a supply list, and laboratory exercises, instructions, and an answer key as well as job aids.
A microscope is a very important instrument in the laboratory. It is necessary to focus your microscope when viewing specimens to perform a proper analysis and give an accurate diagnosis. This job aid gives a brief overview of how to focus on a specimen using a compound microscope.
Video Description: This video demonstrates the proper technique for forward pipetting. This video demonstrates how a laboratory professional transfers 500 microliters of liquid from one buffer to another (buffer A to buffer B) using a 100 to 1000 microliter micropipette.
Safe Use of a BSC depicts best practices to work safely in a BSC.
This video provides a brief overview of the best practices for safely working with formaldehyde and glutaraldehyde in the laboratory.
To make an accurate diagnosis, it is necessary to visualize samples as clearly as possible. Köhler illumination is a light microscopy where the user aligns the illuminating optics to produce a uniform background, maximize contrast, minimize artifacts, and reduce stray light.
The goal of this product is to provide laboratory supervisors and staff with an interactive workbook to share safety protocols and common work practices in the laboratory.
The goal of this product is to provide laboratory supervisors and staff with an interactive workbook to share safety protocols and common work practices in the laboratory.
This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, and the proper response to incidents.
Summary of characteristics (with images) that can be used to identify listeria monocytogenes.
Demonstration of performing a direct smear from a specimen in the last step for culturing microorganisms.
Demonstration of performing a gram stain procedure following the American Society of Microbiology’s Manual of Clinical Microbiology Procedures.
This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, and the proper response to incidents.
This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, waste disposal best practices, and the proper response to incidents.
Video Description: Micropipettes are crucial laboratory instruments used to measure and transfer small volumes of liquid. They are suitable for various applications, such as molecular biology, biochemistry, and cell culture.