Flowchart of Gram stain morphology that can be used to identify aerobic Gram-positive cocci.
Aerobic Gram-Positive Rods Flowchart.
Flowchart (with images) distinguishing alpha and gamma-hemolytic streptococci on blood agar plates.
Summary of characteristics (with images) that can be used to distinguish alpha streptococci from streptococcus pneumoniae on blood agar plates.
Summary of characteristics (with images) that can be used to identify Bacillus spp.
Flowchart that can be used to identify bacteria by using Gram-positive stain.
Table that describes various general media used in a public health or clinical microbiology laboratory.
Guide that facilitators/mentors can use to oversee the completion of basic culture media exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.
This job aid contains a list of terms commonly associated with basic culture media and isolation techniques and a brief description of each term.
This job aid contains hands-on basic culture media and isolation techniques exercises that learners can use along with equipment in their laboratory to reinforce concepts from the associated eLearning course.
This job aid contains a list of supplies needed and instructions for performing a four-quadrant streak on an Agar plate to obtain isolated colonies from a specimen.
This job aid includes examples of recommended media that can be plated to recover microorganisms from a particular body site. The selection of types and forms can vary depending on the laboratory protocol and availability.
Hands-on microscopy laboratory exercises that learners can use along with equipment in their laboratory to reinforce concepts from associated eLearning course.
Microscopes are important pieces of equipment used in the management of patient care. Compound microscopes have mechanical and optical components essential to their function and use. It is important to set up microscopes for optimal viewing every time it is used.
Microscopes are important pieces of equipment used in the management of patient care. Compound microscopes have mechanical and optical components essential to their function and use. It is important to set up microscopes for optimal viewing every time it is used.
Microscopes are important pieces of equipment used in the management of patient care. Compound microscopes have mechanical and optical components essential to their function and use. It is important to set up microscopes for optimal viewing every time it is used.
Compound microscopes require regular cleaning to help ensure accurate diagnoses and prevent damage to the microscope. However, microscopes are delicate instruments that must be handled with care.
A microscope is a very important instrument in the laboratory. It is necessary to focus your microscope when viewing specimens to perform a proper analysis and give an accurate diagnosis. This video gives a brief overview of how to focus on a specimen using a compound microscope.
To make an accurate diagnosis, it is necessary to visualize samples as clearly as possible. Köhler illumination is a light microscopy where the user aligns the illuminating optics to produce a uniform background, maximize contrast, minimize artifacts, and reduce stray light.
The most common type of microscope is a compound light microscope. It contains two or more lenses and uses visible light to produce a two-dimensional image of an object viewed through the oculars.
Size is one of the most important physical features when identifying and characterizing an organism in the lab. The exact size of an organism can be determined using a calibrated ocular micrometer.
Microscopes are important pieces of equipment used in the management of patient care. Compound microscopes have mechanical and optical components essential to their function and use. It is important to set up microscopes for optimal viewing every time it is used.
The creation of RNA is made possible by a process called bacterial transcription. Through transcription the information contained in a section of DNA is replicated to form a new piece of messenger RNA (mRNA). This video will cover the three steps to create mRNA.
Before a cell divides and DNA is passed from one cell to another, a complex process occurs. The DNA strands unwind and separate. Each strand makes a complementary strand by adding the appropriate nucleotides.
Deoxyribonucleic acid (DNA) is a complex molecule of many components. These components can be divided into four main groups: basic elements, nucleosides, nucleotides, and nucleic acids. This video will describe how these four groups build upon each other to create the DNA molecule.
RNA is similar in structure to DNA but is involved in different cellular functions. RNA contains the same basic elements of DNA but with three major differences in its structure. This video will describe these three differences.
In performing molecular procedures in the laboratory, it is essential that you keep contamination down to a minimum. Contamination can affect the results of your test. This video will discuss the necessary steps that should be used in reducing contamination.
Column-based extraction is a method that employs selective binding of nucleic acid to a solid matrix, such as silica that is packed in a column. This video will show this procedure to remove nucleic acid from a specimen.
Gel electrophoresis is a method used for separation of nucleic acid using a porous gel matrix depending on the size of the nucleic acid. This video will show how this procedure is performed.
When performing liquid phase nucleic acid extraction, a method called alcoholic precipitation is used where contaminants such as organic solvents salts and proteins are removed from nucleic acid in a solution. This video will show how the procedure is performed.
Magnetic bead-based extraction is a method that utilizes small particles with a paramagnetic core that binds to nucleic acid. This video will show how the procedure is performed.
Organic extraction is a method used to separate nucleic acid and other cellular components based on their differential solubility. This method uses a mixture of organic solvents, phenol and chloroform, to extract unwanted cellular components from nucleic acid.
Polymerase chain reaction or PCR is a technique for amplifying specific DNA fragments from a DNA template. PCR happens in three basic steps: denaturation, annealing, and extension. This video will show the steps in amplifying specific DNA fragments for the PCR technique.
Detection of PCR products in real time can be accomplished by using fluorescent dyes or probes. Fluorescently labeled probes detect the amount of specific double-stranded DNA sequences.
Flowchart (with images) that can be used to identify beta-hemolytic streptococci on blood agar plates.
Images of positive and negative biochemical tests for Gram negative organisms.
Images showing biochemical tests for identifying Gram- positive organisms.
Guide that facilitators/mentors can use to oversee the completion of Gram-negative laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.
Hands-on Gram-negative exercises that learners can use along with equipment in their laboratory to reinforce concepts from associated eLearning course.
Guide that facilitators/mentors can use to oversee the completion of Gram-positive laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.
This resource familiarizes laboratory professionals with how to read a Gram stain, colonial characteristics, biochemical tests used to identify Gram-positive microorganisms, and commonly used testing algorithms.
Size is one of the most important physical features when identifying and characterizing an organism in the lab. The exact size of an organism can be determined using a calibrated ocular micrometer.
Instructions (with images) for care and maintenance of a microscope.
List of questions to consider while following a specimen from collection through microbiological testing.
Descriptions (with images) showing colony morphology characteristics.
This template is intended to help supervisors or training evaluators assess PPE competency in clinical and public health laboratory settings.
This job aid reviews basic information about diagnostic sensitivity and specificity for clinical laboratory testing.
This resource guide includes available biological, chemical, and radiological resources for laboratories to reference during an emergency.
Flowchart for identifying E. coli (non-stool).
Guide that facilitators/mentors can use to oversee the completion of basic microscopy laboratory exercises. The guide contains objectives, laboratory setup, a supply list, and laboratory exercises, instructions, and an answer key as well as job aids.
A microscope is a very important instrument in the laboratory. It is necessary to focus your microscope when viewing specimens to perform a proper analysis and give an accurate diagnosis. This job aid gives a brief overview of how to focus on a specimen using a compound microscope.
Video Description: This video demonstrates the proper technique for forward pipetting. This video demonstrates how a laboratory professional transfers 500 microliters of liquid from one buffer to another (buffer A to buffer B) using a 100 to 1000 microliter micropipette.
Glossary of terms and references for the Gram Negative Organism ID course.
List of supplies and reagents needed to conduct a Gram stain and instructions on how to conduct a Gram staining procedure.
Images depicting Gram stain results and cell shapes and arrangements.
Flowchart for identifying lactose negative Gram-negative rods.
Flowchart for identifying lactose positive Gram-positive rods.
Gram-Negative Rods Non-Stool Pathogens Flowchart.
Flowchart for identifying Gram-negative rods stool pathogens.
This microlearning focuses on the laboratory technique known as the Gram stain, which is commonly used to differentiate between bacterial species that are Gram-positive and Gram-negative.
This microlearning focuses on the laboratory technique known as the catalase test, which is commonly used to differentiate between bacterial species you will learn the proper techniques with the slide method and the tube method.
This microlearning focuses on the laboratory technique known as the Motility Test, which is commonly used to differentiate between bacterial species by detecting if a bacterium is motile due to the presence of flagella.
This microlearning teaches the proper techniques to be used while performing the Oxidase Test, which is one of several tests that is used to identify Gram-negative bacteria.
Instructions and list of supplies (with images) for examining specimens for yeast.
To make an accurate diagnosis, it is necessary to visualize samples as clearly as possible. Köhler illumination is a light microscopy where the user aligns the illuminating optics to produce a uniform background, maximize contrast, minimize artifacts, and reduce stray light.
Summary of characteristics (with images) that can be used to identify listeria monocytogenes.
This template is intended to help supervisors document the PPE training of employees in clinical and public health laboratory settings. Employees must be trained on PPE usage before they are allowed to perform work in areas requiring PPE.
This template is intended to help supervisors or evaluators of PPE programs in clinical and public health laboratory settings. The goal is to determine whether appropriate policies and procedures are in place to enable employees to safely and effectively use PPE.
Instructions and list of supplies (with images) for preparing a wet mount.
Demonstration of performing a direct smear from a specimen in the last step for culturing microorganisms.
Demonstration of performing a gram stain procedure following the American Society of Microbiology’s Manual of Clinical Microbiology Procedures.
Demonstration of the proper technique for performing an India ink stain.
Demonstration of how to perform a potassium hydroxide or, KOH preparation, for detecting the presence of yeast in a specimen under a microscope.
Demonstration of how to perform a wet mount using a bright-field microscope and the proper equipment.
Guide that facilitators/mentors can use to oversee the completion of routine microscopy procedures laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.
This job aid contains hands-on routine microscopy exercises that learners can use along with equipment in their laboratory to reinforce concepts from the associated eLearning course.
This job aid will familiarize learners with the major sections of the microscope.
Instructions and list of supplies (with images) describing the process of making a smear preparation.
Flowchart (with images) that can be used to identify staphylococci.
Chart and images that can be used to interpret TSI reactions.
Video Description: Micropipettes are crucial laboratory instruments used to measure and transfer small volumes of liquid. They are suitable for various applications, such as molecular biology, biochemistry, and cell culture.
Yersinia pestis Characteristics Chart.
Yersinia pestis Differentiation Chart.
Flowchart for identifying Y. pestis.