Preparedness and Response

Chart listing characteristics of Bacillus Anthracis (Anthrax).

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Flowchart for identifying aerobic Gram-negative rods or Gram-negative coccobacilli.

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Flowchart of Gram stain morphology that can be used to identify aerobic Gram-positive cocci.

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Aerobic Gram-Positive Rods Flowchart.

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This job aid aims to provide lab audiences with general knowledge and tools to conduct a Hotwash and After-Action Report after an incident, near miss, training exercise, or emergency response event.

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Flowchart (with images) distinguishing alpha and gamma-hemolytic streptococci on blood agar plates.

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Summary of characteristics (with images) that can be used to distinguish alpha streptococci from streptococcus pneumoniae on blood agar plates.

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Form used to provide an overview of important points to consider when conducting business from an Emergency Relocation Site.

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Job aid for the specific steps needed to safely attach the regulator to a compressed gas cylinder.

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Chart listing characteristics of B. mallei.

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Flowchart used to identify or rule out B. mallei.

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Chart listing characteristics of B. pseudomallei.

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Flowchart used to rule out or refer B. pseudomallei.

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Flowchart used to rule out or refer Bacillus anthracis.

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Summary of characteristics (with images) that can be used to identify Bacillus spp.

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Flowchart that can be used to identify bacteria by using Gram-positive stain.

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Table that describes various general media used in a public health or clinical microbiology laboratory.

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Guide that facilitators/mentors can use to oversee the completion of basic culture media exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.

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This job aid contains a list of terms commonly associated with basic culture media and isolation techniques and a brief description of each term.

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This job aid contains hands-on basic culture media and isolation techniques exercises that learners can use along with equipment in their laboratory to reinforce concepts from the associated eLearning course.

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This job aid contains a list of supplies needed and instructions for performing a four-quadrant streak on an Agar plate to obtain isolated colonies from a specimen.

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This job aid includes examples of recommended media that can be plated to recover microorganisms from a particular body site. The selection of types and forms can vary depending on the laboratory protocol and availability.

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Hands-on microscopy laboratory exercises that learners can use along with equipment in their laboratory to reinforce concepts from associated eLearning course.

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Microscopes are important pieces of equipment used in the management of patient care. Compound microscopes have mechanical and optical components essential to their function and use. It is important to set up microscopes for optimal viewing every time it is used.

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Microscopes are important pieces of equipment used in the management of patient care. Compound microscopes have mechanical and optical components essential to their function and use. It is important to set up microscopes for optimal viewing every time it is used.

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Microscopes are important pieces of equipment used in the management of patient care. Compound microscopes have mechanical and optical components essential to their function and use. It is important to set up microscopes for optimal viewing every time it is used.

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Compound microscopes require regular cleaning to help ensure accurate diagnoses and prevent damage to the microscope. However, microscopes are delicate instruments that must be handled with care.

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A microscope is a very important instrument in the laboratory. It is necessary to focus your microscope when viewing specimens to perform a proper analysis and give an accurate diagnosis. This video gives a brief overview of how to focus on a specimen using a compound microscope.

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To make an accurate diagnosis, it is necessary to visualize samples as clearly as possible. Köhler illumination is a light microscopy where the user aligns the illuminating optics to produce a uniform background, maximize contrast, minimize artifacts, and reduce stray light.

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The most common type of microscope is a compound light microscope. It contains two or more lenses and uses visible light to produce a two-dimensional image of an object viewed through the oculars.

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Size is one of the most important physical features when identifying and characterizing an organism in the lab. The exact size of an organism can be determined using a calibrated ocular micrometer.

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Microscopes are important pieces of equipment used in the management of patient care. Compound microscopes have mechanical and optical components essential to their function and use. It is important to set up microscopes for optimal viewing every time it is used.

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The creation of RNA is made possible by a process called bacterial transcription. Through transcription the information contained in a section of DNA is replicated to form a new piece of messenger RNA (mRNA). This video will cover the three steps to create mRNA.

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Before a cell divides and DNA is passed from one cell to another, a complex process occurs. The DNA strands unwind and separate. Each strand makes a complementary strand by adding the appropriate nucleotides.

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Deoxyribonucleic acid (DNA) is a complex molecule of many components. These components can be divided into four main groups: basic elements, nucleosides, nucleotides, and nucleic acids. This video will describe how these four groups build upon each other to create the DNA molecule.

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RNA is similar in structure to DNA but is involved in different cellular functions. RNA contains the same basic elements of DNA but with three major differences in its structure. This video will describe these three differences.

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In performing molecular procedures in the laboratory, it is essential that you keep contamination down to a minimum. Contamination can affect the results of your test. This video will discuss the necessary steps that should be used in reducing contamination.

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Column-based extraction is a method that employs selective binding of nucleic acid to a solid matrix, such as silica that is packed in a column. This video will show this procedure to remove nucleic acid from a specimen.

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Gel electrophoresis is a method used for separation of nucleic acid using a porous gel matrix depending on the size of the nucleic acid. This video will show how this procedure is performed.

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When performing liquid phase nucleic acid extraction, a method called alcoholic precipitation is used where contaminants such as organic solvents salts and proteins are removed from nucleic acid in a solution. This video will show how the procedure is performed.

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Magnetic bead-based extraction is a method that utilizes small particles with a paramagnetic core that binds to nucleic acid. This video will show how the procedure is performed.

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Organic extraction is a method used to separate nucleic acid and other cellular components based on their differential solubility. This method uses a mixture of organic solvents, phenol and chloroform, to extract unwanted cellular components from nucleic acid.

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Polymerase chain reaction or PCR is a technique for amplifying specific DNA fragments from a DNA template. PCR happens in three basic steps: denaturation, annealing, and extension. This video will show the steps in amplifying specific DNA fragments for the PCR technique.

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Detection of PCR products in real time can be accomplished by using fluorescent dyes or probes. Fluorescently labeled probes detect the amount of specific double-stranded DNA sequences.

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Flowchart (with images) that can be used to identify beta-hemolytic streptococci on blood agar plates.

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Images of positive and negative biochemical tests for Gram negative organisms.

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Images showing biochemical tests for identifying Gram- positive organisms.

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Guide that facilitators/mentors can use to oversee the completion of Gram-negative laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.

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Hands-on Gram-negative exercises that learners can use along with equipment in their laboratory to reinforce concepts from associated eLearning course.

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Guide that facilitators/mentors can use to oversee the completion of Gram-positive laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.

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This resource familiarizes laboratory professionals with how to read a Gram stain, colonial characteristics, biochemical tests used to identify Gram-positive microorganisms, and commonly used testing algorithms.

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Useful resources to establish or strengthen biosafety practices in a clinical or public health laboratory.  

Biosafety Resources & Tools (cdc.gov)

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This job aid details the use of standard precautions when cleaning up blood or other body fluids, including handwashing, rinsing of mucous membrane or eyes after exposure, treating skin punctures, and reporting exposure, as well as glove safety.

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This job aid can help guide employee conversations with their supervisor for specific information or guidance concerning training, administrative controls, and incident reporting.

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This job aid is a reference document for the required steps to take following a blood or OPIM exposure incident.

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This job aid is a reference document for items that should be included in a biological spill kit and the appropriate steps for cleaning up a blood or OPIM spill.

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Chart listing characteristics of Brucella spp.

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Flowchart used to rule out or refer Brucella spp.

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Size is one of the most important physical features when identifying and characterizing an organism in the lab. The exact size of an organism can be determined using a calibrated ocular micrometer.

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Instructions (with images) for care and maintenance of a microscope.

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Compressed Gas Association Valve Outlet Listing

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Job aid for the steps needed to safely check the compressed gas cylinder for leaks.

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Checklist of activities/tasks needed to safely use biological safety cabinets.

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Featuring the top questions asked during OneLab’s popular 2023 webinar, with answers from Penny Keller, BS MB(ASCP), Centers for Medicare and Medicaid Services.

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List of questions to consider while following a specimen from collection through microbiological testing.

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Descriptions (with images) showing colony morphology characteristics.

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This job aid details the use of standard disinfectants and antiseptics in the laboratory, including instructions for the preparation of a 10% bleach solution.

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This job aid contains personal safety work and general safety work practices for working in the Laboratory.

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This template is intended to help supervisors or training evaluators assess PPE competency in clinical and public health laboratory settings.

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Worksheet to list the various communications systems a laboratory uses, the current providers, restoration contacts, and backup modes of communication.

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A notification list used to determine who has the authority to activate the continuity of operations plan based on predetermined triggers and how others in the organization will be notified of the decision to activate.

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Worksheet to list all facilities in which the organization operates, and designations for which facilities will be needed in an emergency or disaster situation.

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Worksheet to list essential functions, personnel needed, and other important information needed to maintain the continuity of the essential function when moving to an alternate worksite.

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Flowchart to assist in determining whether to activate a continuity of operations plan.

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Worksheet to list an organization’s critical functions, the primary authority (e.g., decision-making, procurement), and the order of succession in case the primary authority is unavailable.

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Worksheet to ensure that secure methods of communications are available in a continuity of operations situation.

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Guide for recording priorities and essential functions for laboratory continuity of operations.

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Worksheet to determine overall staffing needs for essential laboratory functions.

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Instructions, guidance, and sample text for the development of continuity plans and programs.

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This infographic includes general information to improve COVID-19 specimen handling and processing for more accurate results.

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Protocol for labeling secondary laboratory containers.

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This product includes crisis response information and resources for laboratories to use in the event of various crisis events.

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Table used to determine whether to activate a continuity of operations plan based on the impact various emergencies may have on the laboratory.

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This resource discusses what virtual training is and explores the features of popular online learning platforms.

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Template that planners can use to detail how essential laboratory operations will continue in the event the primary operating facility is incapacitated and personnel are unavailable or incapable of deploying to the alternate facility.

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This job aid reviews basic information about diagnostic sensitivity and specificity for clinical laboratory testing.

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The Diagnostic Stewardship toolkit was created to help organizations form diagnostic stewardship teams and apply guiding principles. Diagnostic Stewardship supports ordering the right test for the right patient at the right time to improve patient outcomes.   

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Chart listing characteristics of biothreat agents and similar organisms.

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Table listing characteristics of biothreat agents and similar organisms.

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Chart differentiating Brucella spp. from other urea-positive, oxidase-positive gram-negative coccobacilli.

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Donning and doffing Personal Protective Equipment is essential to keeping laboratorians and the public safe from possible contamination. This video focuses on donning and doffing a laboratory coat, gloves, and safety glasses with wrap around sides.

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This video demonstrates the donning and doffing of the following PPE: Gown, Gloves, Disposable Face Mask, and Face Shield. The doffing portion of this video demonstrates removing the gown and gloves.

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This video demonstrates the donning and doffing of the following PPE: Gown, Gloves, Disposable Face Mask, and Face Shield. The doffing portion of this video demonstrates removing the gown and gloves.

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This job aid is based on the fictitious EdU scenario in the Laboratory Risk Management course and is intended only for the purpose of this risk management training. Please reference your laboratory's policies and procedures for site-specific risks and considerations.

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Table to list contact information of emergency personnel and other important contacts.

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This resource guide includes available biological, chemical, and radiological resources for laboratories to reference during an emergency.

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This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, and the proper response to incidents.

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Flowchart for identifying E. coli (non-stool).

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Examples of statements of applicability and scope for continuity of operations plans.

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Examples of executive summaries for continuity of operations plans.

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Examples of purpose statements for laboratory continuity of operations plans.

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Guide that facilitators/mentors can use to oversee the completion of basic microscopy laboratory exercises. The guide contains objectives, laboratory setup, a supply list, and laboratory exercises, instructions, and an answer key as well as job aids.

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A microscope is a very important instrument in the laboratory. It is necessary to focus your microscope when viewing specimens to perform a proper analysis and give an accurate diagnosis. This job aid gives a brief overview of how to focus on a specimen using a compound microscope.

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This is a sample formaldehyde job hazard analysis that can be used to document the chemical’s hazards and controls by job step. It also contains information about emergency procedures, training, and review and monitoring.

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Video Description: This video demonstrates the proper technique for forward pipetting. This video demonstrates how a laboratory professional transfers 500 microliters of liquid from one buffer to another (buffer A to buffer B) using a 100 to 1000 microliter micropipette.

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Descriptions and images depicting characteristics of Francisella tularensis.

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Flowchart describing major characteristics in identifying Francisella tularensis.

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Good Work Practices demonstrates the performance checks, the proper placement of equipment, and additional tasks needed to ensure a good performance of chemical fume hoods.

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How Airflow is Affected demonstrates how airflow in a chemical fume hood can be interrupted, altered, and changed by different factors.

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Some laboratory procedures require the use of compressed gas cylinders, and it’s important to learn how to safely and properly use them.

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Knowing how to properly use compressed gas cylinders is essential for maintaining a safe laboratory. The regulator on a gas cylinder controls the flow and pressure of gas from the cylinder to your laboratory equipment.

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Checking your compressed gas cylinder for leaks will help keep your laboratory safe and will also help maintain accurate laboratory procedures. This training video shows how to use a bubble test to check for leaks and changes in gas pressure at both the inlet and outlet valves on the regulator.

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Incidents in the laboratory are situations in which an employee is injured or exposed to a pathogen. An incident without an injury is known as a near miss. To reduce risk, laboratories should put various safety controls in place, including the best practice of reporting all incidents.

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A laboratory incident, such as a sharps injury, could cause an employee serious harm or exposure to a dangerous pathogen. A near miss is an incident that doesn’t result in an injury or exposure, it’s called a near miss.

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Biological, chemical, and physical hazards can put laboratory employees at risk for safety incidents that could injure themselves, fellow employees, the public, and the environment.

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Laboratory employees are at risk for coming into contact with biological, chemical, and physical hazards in their daily work.

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Working in a laboratory can put an employee at risk for coming in contact with biological, chemical, and physical, and electrical hazards.

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Directional airflow in the laboratory is an engineering control that keeps possibly contaminated air in a laboratory from reaching other areas. The constant influx of air directs flow through biological safety cabinets and fume hoods where the air can travel through filters.

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Standard operating procedures (SOPs) are administrative controls that help reduce risk from harm by identifying hazards in the laboratory.

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Wearing proper personal protective equipment (PPE), like a laboratory coat, can help protect employees from incidents like spilling or splashing biological or chemical hazards on themselves while performing procedures in the laboratory.

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Methylene chloride, a chemical used in many laboratory procedures, is a potential cancer-causing chemical that’s been known to harm the brain, liver, and heart after repeated exposure to large volumes.

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Continual improvement means making things better to benefit both the customer and the laboratory.

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The cost of quality is more than a management tool. It’s a mindset that should be adopted at every operational level of a laboratory. The implications and cost associated with poor quality, or no quality, create ripple effects well beyond the walls of your laboratory.

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Clinical and public health laboratories have a responsibility to ensure all specimen and testing activities are of the highest quality. Quality in the laboratory is a continual process and best accomplished using a systematic, managed approach.

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Investing in a QMS will help your laboratory efficiently manage organizational assets, quantify the value, and impact a QMS brings, and identify opportunities to continuously improve your laboratory’s mission.

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Reference Document for the 12 Quality System Essentials (QSE), that describe the foundational elements of laboratory Quality Management Systems (QMS). 

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Cleaning Up a Spill demonstrates how to appropriately clean up a spill inside a biosafety cabinet.

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Completing Work in a BSC demonstrates the steps to be performed after BSC work is completed.

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Factors Affecting Airflow Inside the BSC demonstrates how Airflow in a biological safety cabinet or BSC can be interrupted, altered, and changed by many different factors. These interruptions can lead to potential exposures and/or product contamination.

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Preparing for Work in a BSC demonstrates the performance checks, the proper placement of equipment, and additional tasks needed to prepare for working inside of a biological safety cabinet.

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Safe Use of a BSC depicts best practices to work safely in a BSC.

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This video provides a brief overview of the best practices for safely working with formaldehyde and glutaraldehyde in the laboratory.

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Fact sheet listing common questions and answers about genetic testing and results.

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This job aid details glove safety in the laboratory, including how to choose disposable gloves, precautions while wearing protective gloves, and the safe removal and disposal of gloves.

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This is a sample glutaraldehyde job hazard analysis that can be used to document the chemical’s hazards and controls by job step. It also contains information about emergency procedures, training, and review and monitoring.

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Glossary of terms and references for the Gram Negative Organism ID course.

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List of supplies and reagents needed to conduct a Gram stain and instructions on how to conduct a Gram staining procedure.

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Images depicting Gram stain results and cell shapes and arrangements.

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Flowchart for identifying lactose negative Gram-negative rods.

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Flowchart for identifying lactose positive Gram-positive rods.

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Gram-Negative Rods Non-Stool Pathogens Flowchart.

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Flowchart for identifying Gram-negative rods stool pathogens.

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This job aid details the steps for proper hand hygiene in the laboratory, including instructions for cleaning the hands with water or with an alcohol-based sanitizer.

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Table listing examples of potential laboratory threats (e.g., earthquake or fire), mitigation measures, and risks.

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Fact sheet containing basic information about molecular genetic testing and results.

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This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, and the proper response to incidents.

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This document contains the qualifications required for a clinical consultant in a laboratory performing high complexity testing.

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This document contains the qualifications required for a cytology general supervisor in a laboratory performing high complexity testing.

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This document contains the qualifications required for a cytotechnologist in a laboratory performing high complexity testing.

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This document contains the qualifications required for a general supervisor in a laboratory performing high complexity testing.

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This document contains the qualifications required for a laboratory director in a laboratory performing high complexity testing.

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This document contains the qualifications required for a technical supervisor in a laboratory performing high complexity testing. Cytology technical supervisor qualifications are also included in the cytology section.

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This document contains the qualifications required for testing personnel in a laboratory performing high complexity testing.

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The LIMS manages the information workflow through the laboratory. It interfaces or interacts with many of the other systems within and outside the laboratory. These systems can include those that interface with laboratory instruments.

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This microlearning focuses on the laboratory technique known as the Gram stain, which is commonly used to differentiate between bacterial species that are Gram-positive and Gram-negative.

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This microlearning focuses on the laboratory technique known as the catalase test, which is commonly used to differentiate between bacterial species you will learn the proper techniques with the slide method and the tube method.

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This microlearning focuses on the laboratory technique known as the Motility Test, which is commonly used to differentiate between bacterial species by detecting if a bacterium is motile due to the presence of flagella.

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This microlearning teaches the proper techniques to be used while performing the Oxidase Test, which is one of several tests that is used to identify Gram-negative bacteria.

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Worksheet to help identify essential and non-essential laboratory functions.

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This resource discusses what learning engagement is, the benefits that come from it, and the practices that you can take to increase learner engagement.

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Instructions for, and examples of, logging or recording results for qualitative tests, quantitative tests, quality control – qualitative tests, quality control – quantitative tests, and multiple tests.

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eLearning course to equip learners with foundational information about CLIA.

 

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To understand how test results are obtained, you need to start from the beginning where specimens are collected. This video will give an overview of specimen collection, testing, and test results.

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Data and reports may be sent to stakeholders outside the laboratory. Some examples of where data may be sent include electronic health records, vital records, and a disease registry. This data will follow at least one of two distinct paths. This video will discuss these two paths.

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Depending on your laboratory certain tests may not be automated. This could be due to the nature of the test or because the testing instrument does not have a direct interface with the LIMS. For example, some tests require visual observation and manual data entry.

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Laboratory data and test results must be properly stored and managed. Proper data storage is a critical component of ensuring data integrity throughout the laboratory process. All data and results must be stored; however, they can live in many places within the laboratory.

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Everyone inside and outside of the laboratory has an important role to play in laboratory informatics. The extent of the role and specific responsibilities will vary. This video will discuss specific roles and responsibilities of those working in the laboratory with laboratory informatics.

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The post analytic phase consists of communicating testing results and storage or disposal of the specimen. This video will describe how the LIMS is involved in communicating results and determining storage or disposal of the specimen.

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Laboratories play a crucial role in supporting public health. Accurate diagnoses of diseases and protection from health threats depend on a laboratory's ability to produce quality data. There are many areas in the life of a specimen workflow where data quality can be compromised.

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Throughout the life of a specimen, the amount of data associated with a specimen increases creating various relationships made up of different types of data. This video will cover the different types of data captured in the LIMS, their relationship, and importance to each other.

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Data standards provide a method to convey information in valid, meaningful, and actionable ways. Although there are many types of data standards, in this video we will focus on data representation and data exchange standards.

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The last step in the analytic process is recording laboratory test results. After testing, results are either recorded manually or through automation into the LIMS. During this process it is imperative that the data is checked for accuracy to ensure incorrect data is not entered into the LIMS.

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A LIMS helps laboratories track and manage the workflow of a specimen and its associated data from the time a specimen is received through the reporting of results. LIMS have many capabilities and benefits. In this video we will describe the capabilities, benefits, and limitations of a LIMS.

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Informatics is a broad field that encompasses information science, information technology algorithms, and social science. In these videos we will review two types of informatics: laboratory informatics and public health informatics.

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Instructions and list of supplies (with images) for examining specimens for yeast.

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To make an accurate diagnosis, it is necessary to visualize samples as clearly as possible. Köhler illumination is a light microscopy where the user aligns the illuminating optics to produce a uniform background, maximize contrast, minimize artifacts, and reduce stray light.

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Clinical and public health laboratories can use this toolkit to translate complex information into user-friendly messages for various audiences.

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This job aid covers essential topics for correctly delegating responsibilities to CLIA laboratory personnel and for identifying responsibilities a laboratory director must retain.

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An infographic and summary that depicts the Laboratory Information Management System (LIMS) as a “system of systems”.

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Manual summarizing rules and guidelines for using a standardized abstraction form to record evidence for systematic reviews for best practices in laboratory medicine.

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The goal of this product is to provide laboratory supervisors and staff with an interactive workbook to share safety protocols and common work practices in the laboratory.

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The goal of this product is to provide laboratory supervisors and staff with an interactive workbook to share safety protocols and common work practices in the laboratory.

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This Laboratory Onboarding Template (LOT) is a central location for laboratory-specific information and foundational training resources to rapidly onboard new hires or surge support staff from multidisciplinary backgrounds.

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The Laboratory Personal Protective Equipment (PPE) Toolkit is a guide to resources on using PPE in clinical and public health laboratories.

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This quick guide contains links to the resources included in the Laboratory Personal Protective Equipment (PPE) Toolkit.

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The Supply Chain Frequently Asked Questions Job Aid is a follow-up resource from the “Supply Chain Lessons Learned and Q&A” OneLab network event held on October 28, 2021. 

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This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, and the proper response to incidents.

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Blank results log for quantitative tests.

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Summary of characteristics (with images) that can be used to identify listeria monocytogenes.

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This flowchart outlines the qualifications necessary for a clinical consultant to comply with CLIA regulations related to moderate complexity testing as specified in 42 CFR 493.1417.

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This flowchart outlines the qualifications necessary for a laboratory director to manage and direct laboratory personnel and the performance of moderate complexity tests as specified by CLIA regulations in 42 CFR 493.1405.

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This flowchart details the qualifications required for a technical consultant to ensure compliance with CLIA regulations for moderate complexity testing as specified in 42 CFR 493.1411.

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This infographic is a resource/tool to improve nasal mid-turbinate swab specimen collection for SARS-CoV-2 testing.

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This infographic is a resource/tool to improve nasopharyngeal swab specimen collection for SARS-CoV-2 testing.

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Worksheet to list individuals and organizations to notify in the event of an emergency and the person responsible for notifying them.

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Worksheet to notify staff and others when the laboratory’s continuity of operations plan is implemented.

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In this scenario, the user will demonstrate the proper steps to safely load, operate, and unload an autoclave. The user is tasked with decontaminating biohazardous waste. The autoclave scenario is performed in the Autoclave room.

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In this scenario, the user is tasked with centrifuging specimens with a swinging bucket centrifuge. The user must select the correct tubes, properly balance, and load the swinging bucket centrifuge.

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In this scenario, the user is tasked with packing and next-day air shipping a Category B specimen of Salmonella during a nationwide outbreak and sending it to another laboratory for further evaluation.

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Worksheet to list essential functions, key personnel, and their successors.

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This job aid details the steps to follow when performing external control testing using known positive or negative clinical samples. It includes steps to follow.

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This template is intended to help supervisors document the PPE training of employees in clinical and public health laboratory settings. Employees must be trained on PPE usage before they are allowed to perform work in areas requiring PPE.

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This template is intended to help supervisors or evaluators of PPE programs in clinical and public health laboratory settings. The goal is to determine whether appropriate policies and procedures are in place to enable employees to safely and effectively use PPE.

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Table to list names and contact information for each employee.

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The Point of Care Testing (POCT) Personal Protective Equipment (PPE) Toolkit is a guide to resources on using PPE in POCT settings.

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Instructions and list of supplies (with images) for preparing a wet mount.

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Flowchart used to rule out or refer Francisella tularensis.

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Tool to help identify and prioritize essential laboratory functions.

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Regulatory requirements, resources including forms and examples, and images of common microscopic findings for the nine specific microscopic examinations that may be performed under a Certificate of PPM Procedures.

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This job aid details the steps to follow when performing quality control testing to ensure tests perform as expected and alert the user when problems occur.

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This form will be used to submit resources to OneLab REACH and include the details for requirements

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Worksheet to record changes to a continuity of operations plan outside the official plan review cycle.

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Job aid for the specific steps needed to safely remove the regulator from a compressed gas cylinder.

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Guidance for strengthening the culture of safety in the laboratory as it pertains to reporting incidents and near misses.

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This job aid is a downloadable and printable results log for reporting qualitative testing.

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This job aid is a downloadable and printable results log for reporting quantitative testing with quality control.

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This job aid is a downloadable and printable results log for reporting multiple types of testing.

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This job aid is a downloadable and printable results log for reporting qualitative testing with quality control.

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This job aid is a downloadable and printable results log for reporting quantitative testing with quality control.

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Risk Matrix Example

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Demonstration of performing a direct smear from a specimen in the last step for culturing microorganisms.

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Demonstration of performing a gram stain procedure following the American Society of Microbiology’s Manual of Clinical Microbiology Procedures.

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Demonstration of the proper technique for performing an India ink stain.

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Demonstration of how to perform a potassium hydroxide or, KOH preparation, for detecting the presence of yeast in a specimen under a microscope.

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Demonstration of how to perform a wet mount using a bright-field microscope and the proper equipment.

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Guide that facilitators/mentors can use to oversee the completion of routine microscopy procedures laboratory exercises. The guide contains instructions for laboratory exercises, objectives, laboratory setup, supply list, job aids, and an answer key.

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This job aid contains hands-on routine microscopy exercises that learners can use along with equipment in their laboratory to reinforce concepts from the associated eLearning course.

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This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, and the proper response to incidents.

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This is a sample chemical job hazard analysis that can be used to document a chemical’s hazards, volume, activities, exposure routes, and control measures volumes of specific chemicals used in the laboratory. Information on dichloromethane (methylene chloride) is used as an example.

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This job aid serves as a quick reference guide for personnel trained to pack and ship suspected or confirmed SARS-CoV-2 specimens.

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This job aid will familiarize learners with the major sections of the microscope.

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This job aid is a downloadable and printable example of a checklist that details four main areas of focus when completing pretesting tasks: 1. preparation of the work area; 2. checking and recording temperatures; 3. maintaining equipment; and 4. preparing materials for testing.

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Job aid for the specific steps needed to safely set the regulator pressure for a compressed gas cylinder.

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Example of typical shipping papers used for ground transport of Category A substances.

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Job aid for the specific steps needed to safely shut down and remove the regulator on a compressed gas cylinder.

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SME Top 10 Presentation Tips

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Instructions and list of supplies (with images) describing the process of making a smear preparation.

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This job aid defines the criteria to properly ship biological specimens to the Centers for Disease Control and Prevention (CDC).

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Flowchart (with images) that can be used to identify staphylococci.

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Decision tree to help classify substance if shipping by motor vehicle courier/ground in accordance with Hazardous Materials Regulations (HMR).

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Decision tree to help determine the classification for substance if shipping by air.

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Reference document for packing Category A, Category B, and Exempt Human and Exempt Animal Specimens.

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Reference table for labeling, marking, and documenting requirements for the various classifications and modes of transport.

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Checklist containing study quality components.

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Table summarizing recommended biosafety levels for infectious agents.

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This job aid details the steps to follow when performing and logging temperature control checks in the laboratory. It includes instructions for recording temperatures and an example of a completed monthly refrigerator/freezer temperature log.

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Worksheet that planners can use to list testing, training, and exercise requirements and the frequency that each should occur.

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eLearning course to prepare physicians, midlevel providers, and dentists to meet requirements for PPM procedures under a CLIA certificate. 

 

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This job aid provides tips for Selecting and Using Personal Protective Equipment (PPE).

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Fact sheet containing recommendations for laboratory professionals performing molecular genetic testing.

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Chart and images that can be used to interpret TSI reactions.

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Worksheet for recording details about essential files, records, and databases that need to be accessed after the laboratory’s continuity of operations plan has been implemented.

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This job aid introduces clinical and public health laboratory professionals to the hazards they may encounter while working in a clinical or public health laboratory, ways to mitigate risks, waste disposal best practices, and the proper response to incidents.

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Reference for items that are forbidden from being put down the drain.

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Video Description: Micropipettes are crucial laboratory instruments used to measure and transfer small volumes of liquid. They are suitable for various applications, such as molecular biology, biochemistry, and cell culture.

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Yersinia pestis Characteristics Chart.

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Yersinia pestis Differentiation Chart.

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Flowchart for identifying Y. pestis.

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